【文獻題目】  Photoperiod affects Harderian gland morphology and secretion in female Cricetulus barabensis: Autophagy,apoptosis, and mitochondria

【作者】  Zhe Wang, Jin-Hui Xu, Jun-Jie Mou，et al.

【作者單位】  曲阜師范大學(xué)

【文獻中引用的產(chǎn)品】  倉鼠褪黑素（MT）ELISA試劑盒

【關(guān)鍵詞】  photoperiod, Harderian gland, autophagy, apoptosis, mitochondria

【DOI】  doi.org/10.3389/fphys.2020.00408

【影響因子(IF)】  3.22

【出版期刊】   《Frontiers in Physiology》

【產(chǎn)品原文引用】

Sample Preparation

Hamsters were sacrificed by CO2 asphyxiation at 22:00 on the last day, after all hamsters had been in darkness for at least 2 h. Blood samples were collected immediately from the heart after sacrifice,stored at 4?C for 30 min, and then centrifuged at 3000 rpm for 15 min at 4?C. Serum MT levels were estimated using an enzyme-linked immunosorbent assay (Shanghai Hengyuan Biological Technology Co., Ltd., H-40277, Shanghai, China). The HGs were removed, with lengths and weights then recorded. The HGs from five hamsters were fixed by immersion and processed for light and electron microscopy. The right HGs were immersed in 10% buffered formalin for paraffin section embedding, and the left HGs were immersed in glutaraldehyde-paraformaldehyde for transmission electron microscopy (TEM). The remaining HGs from 15 hamsters in each group were frozen in liquid nitrogen and stored in a refrigerator at −80℃ for subsequent Western blotting, real-time polymerase chain reaction (RTPCR), and immunofluorescence histochemical analyses. In each experiment, unilateral HGs from 10 hamsters were used for quantification. All procedures were carried out in accordance with approved guidelines.